GeneRulor | Gene Editing & CGT Safety Assessment Service Provider

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CRISPR Functional Genomics Screening

Industry Background

CRISPR functional genomics screening has become a core tool for drug target discovery, research into drug resistance mechanisms, and gene function annotation. By constructing genome-wide sgRNA libraries to systematically knock out, activate, or inhibit each gene at the cellular level, and combining these with phenotypic screening, the causal relationships between genes and phenotypes can be elucidated in a high-throughput manner. From discovering oncology drug targets and conducting synthetic lethality screens to identifying immune checkpoints and dissecting metabolic pathways, CRISPR screening is accelerating biological discovery and the development of innovative drugs.

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Industry Challenges

Library Design and Construction

○ Genome-scale sgRNA libraries contain a large number of sequences (60,000-100,000), leading to high design and synthesis costs

○ Library uniformity critically impacts screening quality; poor-quality libraries result in false negatives

○ The design principles for activation (CRISPRa) and interference (CRISPRi) libraries differ fundamentally from those for knockout libraries

Screening Execution

○ Large-scale cell culture and library transduction procedures are technically complex

○ Insufficient screening coverage compromises the reliability of results

○ Long screening timelines and difficulties in optimizing experimental conditions

Data Analysis

○ Handling large volumes of sequencing data requires complex analytical pipelines

○ Selection and parameter optimization of hit prioritization algorithms (e.g., MAGeCK, BAGEL) are challenging

○ Translating hit lists into verifiable targets demands biological insight

Validation and Translation

○ Single-gene validation of screening hits is time-consuming

○ Translational validation from cellular phenotypes to animal models

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Solution Overview

GeneRulor provides a comprehensive, end-to-end CRISPR screening solution encompassing "Library Design → Library Construction → Screening Execution → Bioinformatics Analysis → Hit Validation." We support three screening modalities: knockout (CRISPRko), activation (CRISPRa), and interference (CRISPRi), empowering our clients to efficiently discover drug targets and functional genes.

Core Services

Off-the-Shelf Genome-Wide Libraries:

○ CRISPR-KO Library – for gene knockout screening

○ CRISPRa Activation Library – for gene overexpression screening

○ CRISPRi Inhibition Library – for gene silencing screening

Custom Libraries

○ Customer-specified gene sets – tailored to specific research needs