GeneRulor pA-Transposome

1. Product Overview

pA-Transposome is a highly efficient transposome complex with pre-embedded adapters, which is pre-assembled from optimally designed pA-Transposase and specific DNA adapters. The transposome is fused with a Protein A domain, allowing it to specifically bind to antibodies and achieve precise chromatin localization. As a ready-to-use reagent, pA-Transposome eliminates the tedious step of in-house transposome assembly by users and greatly simplifies the experimental procedure of CUT&Tag (Cleavage Under Targets and Tagmentation). By binding to specific antibodies, pA-Transposome can be accurately localized to target chromatin regions to realize in situ DNA fragmentation and tagging, which significantly improves the efficiency and accuracy of epigenetic research. Compared with the traditional ChIP-seq technology, the CUT&Tag method using pA-Transposome has distinct advantages of low sample demand, low background signal and high sensitivity, and is particularly suitable for epigenomic analysis at the single-cell level. Its core Protein A domain has universal and potent binding affinity for IgG antibodies (especially the Fc region of IgG) from multiple species such as humans and rabbits, ensuring high efficiency and stability in common experimental systems. This makes the tool particularly suitable for epigenetic research centered on human or rabbit-derived antibodies, such as chromatin analysis of histone modifications. If the experiment mainly uses mouse IgG1 antibodies, the pG-Transposome with a complementary binding profile can be selected for combination, providing users with a flexible and precise solution.

2. Applications

CUT&Tag Technology: pA-Transposome can bind to specific antibodies to achieve precise tagging of regions of interest and library construction. Its working principle is that antibodies recognize specific chromatin marks or DNA-binding proteins, guide transposase to target sites, and realize highly specific DNA fragmentation and adapter ligation. This technology is particularly suitable for studying low-abundance transcription factor binding sites and specific histone modifications, which can significantly improve sequencing efficiency and data quality, reduce background noise, and provide a powerful tool for precise epigenomic analysis.

Figure 1. Experimental flow chart of CUT&Tag using pA-Transposase

References

[1] Kaya-Okur HS, Wu SJ, Codomo CA, Pledger ES, Bryson TD, Henikoff JG, Ahmad K, Henikoff S. CUT&Tag for efficient epigenomic profiling of small samples and single cells. Nat Commun. 2019 Apr 29;10(1):1930. doi: 10.1038/s41467-019-09982-5.

[2] Farzad N, Enninful A, Bao S, Zhang D, Deng Y, Fan R. Spatially resolved epigenome sequencing via Tn5 transposition and deterministic DNA barcoding in tissue. Nat Protoc. 2024 Nov;19(11):3389-3425. doi: 10.1038/s41596-024-01013-y.