dPspCas13b-ADAT2 RNA Editing Nuclease

Product Introduction

dPspCas13b-ADAT2 is an RNA editing tool based on CRISPR technology, serving as a core component of the REPAIR (RNA Editing for Programmable A to I Replacement) system. This fusion protein consists of a catalytically inactive Prevotella sp. P5-125 Cas13b (dPspCas13b) fused with the ADAT2 adenosine deaminase domain, enabling precise base editing at the RNA level and providing a novel strategy for correcting pathogenic RNA mutations.

Cas13b is a type VI-B CRISPR effector protein whose natural function is to target and bind RNA molecules. By introducing key mutations to inactivate its HPN nuclease domains, dPspCas13b completely loses its RNA cleavage activity while fully retaining the ability to specifically bind target RNA sequences guided by CRISPR RNA (crRNA), establishing it as a precise RNA targeting platform. ADAT2 is an adenosine deaminase naturally involved in tRNA modification that catalyzes the hydrolytic deamination of adenosine (A) to inosine (I). Since inosine is recognized as guanosine (G) by the ribosome during translation, A-to-I editing effectively achieves an A-to-G functional alteration.

When the crRNA guides dPspCas13b-ADAT2 to bind to a target transcript, ADAT2 locally deaminates specific adenosine positions, enabling precise RNA sequence recoding.