Fast Tagment DNA Library Prep Kit For MGI

1. Product Overview

Fast Tagment DNA Library Prep Kit is a tailor-made solution for the MGI high-throughput sequencing platform. The kit can efficiently convert DNA samples into dedicated libraries applicable to the MGI sequencing platform. It adopts transposase technology to integrate multiple tedious steps required in traditional methods, such as DNA fragmentation, end repair and adapter ligation, into a single enzymatic reaction process. This technological breakthrough not only greatly reduces the amount of starting DNA required, but also significantly shortens the time needed for library construction.

2. Applications

Fast Tagment DNA Library Prep Kit For MGI leverages its special transposition mechanism to simultaneously complete two steps—DNA fragmentation and adapter ligation—in a single reaction. This "one-step" tagmentation method greatly simplifies the experimental workflow, which not only saves operation time but also reduces experimental costs, making the library construction process more efficient.

Figure 1. Schematic diagram of second-generation sequencing library construction

References

[1] Li N, Jin K, Bai Y, Fu H, Liu L, Liu B. Tn5 Transposase Applied in Genomics Research. Int J Mol Sci. 2020 Nov 6;21(21):8329. doi: 10.3390/ijms21218329.

[2] Zhang H, Lu T, Liu S, Yang J, Sun G, Cheng T, Xu J, Chen F, Yen K. Comprehensive understanding of Tn5 insertion preference improves transcription regulatory element identification. NAR Genom Bioinform. 2021 Oct 27;3(4):lqab094. doi: 10.1093/nargab/lqab094.