FrCas9 Nuclease
Product Introduction
FrCas9 Nuclease is a novel type II-A CRISPR-Cas9 system derived from Faecalibaculum rodentium, adding an important new member to the genome editing toolbox. This protein consists of 1372 amino acids, with its most distinctive feature being its unique palindromic PAM recognition sequence, 5'-NNTA-3'. This concise dinucleotide core PAM not only significantly expands the genomic targeting range but also endows FrCas9 with a special capability: the ability to directly target the TATA-box region within eukaryotic promoters. As a key element of core promoters involved in regulating transcription initiation, the TATA-box makes this characteristic a novel tool for studying TATA-box-related diseases and potential therapeutic interventions.
While maintaining editing efficiency comparable to, or even higher than, SpCas9, FrCas9 exhibits significantly lower off-target effects. Assessed by high-throughput technologies such as GUIDE-seq and AID-seq, the number of off-target sites for FrCas9 is far fewer than for SpCas9, and it shows lower tolerance for mismatches, demonstrating stricter recognition specificity.
Product Specifications
Parameter | Specification |
Source | Recombinant expression in E. coli |
Molecular Weight | ~160 kDa |
Concentration | 20 µM |
PAM Sequence | 5'-NNTA-3' |
Cleavage Product | Blunt-ended DSB |
Purity | ≥95% (SDS-PAGE) |
Endotoxin | <1 EU/μg |
Storage Buffer | 50 mM Tris-HCl, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol |
10× Reaction Buffer | 50 mM Tris-HCl, 100 mM NaCl, 10 mM MgCl₂, 100 μg/ml BSA, pH 7.9 |
Storage Conditions | Long-term storage at -80°C; short-term storage at -20°C |
Product Specifications
Specifications | Catalog Number | Concentration | Volume |
100 pmol | GR100701 | 20 μM | 5 μL |
500 pmol | GR100702 | 20 μM | 25 μL |
2500 pmol | GR100703 | 20 μM | 125 μL |
Application Scenarios
TATA-box Targeting: Directly regulating the expression of TATA-box associated genes.
AT-rich Region Editing: Efficiently editing genomic regions with high AT content.
CRISPRa/i: TATA-box-directed transcriptional regulation.
High-Fidelity Editing: Applications requiring low off-target risk.
Plant Genome Editing: Efficient editing of TA-rich plant genomes.
References
Cui Z, et al. (2022). FrCas9 is a CRISPR/Cas9 system with high editing efficiency and fidelity. Nat Commun. 13(1):1425.
He Y, et al. (2024). Expanding plant genome editing scope and profiles with CRISPR-FrCas9 systems targeting palindromic TA sites. Plant Biotechnol J. 22(9):2488-2503.
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