Fast Tagment DNA Library Prep Kit For Illumina

1. Product Overview

Fast Tagment DNA Library Prep Kit is a tailor-made solution for Illumina high-throughput sequencing platforms. The kit can efficiently convert DNA samples into special libraries suitable for Illumina sequencing platforms. It adopts transposase technology to integrate multiple tedious steps that need to be completed step by step in traditional methods, including DNA fragmentation, end repair and adapter ligation, into a single enzymatic reaction process. This technological breakthrough not only greatly reduces the amount of starting DNA required, but also significantly shortens the time needed for library construction.

2. Product Features

(1) Simplified workflow: Revolutionary transposase technology integrates traditional multi-step operations (DNA fragmentation, end repair and adapter ligation) into a single enzymatic reaction, greatly simplifying the experimental process;

(2) Ultra-low starting amount requirement: Applicable to DNA samples as low as 1 ng, solving the problem of scarce sample processing;

(3) Significantly reduced experimental time: The experimental time is reduced by up to 75% compared with traditional library construction methods;

(4) Flexible specification options: Three starting amount specifications of 50 ng, 5 ng and 1 ng are available to meet different experimental needs;

(5) Excellent stability and repeatability: A strict quality control system ensures the consistency of inter-batch results;

(6) Superior compatibility: Perfectly compatible with the full range of Illumina sequencing platforms;

(7) Easy operation: Fewer experimental steps reduce the risk of operational errors;

(8) High cost-effectiveness: Less reagent consumption and short experimental time improve laboratory efficiency.

3. Applications

Fast Tagment DNA Library Prep Kit For Illumina utilizes its special transposition mechanism to complete two steps of DNA fragmentation and adapter ligation simultaneously in a single reaction. This one-step tagmentation method greatly simplifies the experimental process, not only saving operation time, but also reducing experimental costs, making the library construction process more efficient.

Figure 1. Schematic diagram of second-generation sequencing library construction

References

[1] Li N, Jin K, Bai Y, Fu H, Liu L, Liu B. Tn5 Transposase Applied in Genomics Research. Int J Mol Sci. 2020 Nov 6;21(21):8329. doi: 10.3390/ijms21218329.

[2] Zhang H, Lu T, Liu S, Yang J, Sun G, Cheng T, Xu J, Chen F, Yen K. Comprehensive understanding of Tn5 insertion preference improves transcription regulatory element identification. NAR Genom Bioinform. 2021 Oct 27;3(4):lqab094. doi: 10.1093/nargab/lqab094;