Hybridization Capture Probe SynthesisService

1. Introduction to Probe SynthesisService

The hybridization capture probe synthesis service provided by GeneRulor Co., Ltd. is a professional solution suitable formulti-site detection of target genes. Based on specific probe synthesis technology, it supports efficient capture and enrichment of target regions ingenomic DNA, laying a foundation for high-throughput sequencing. It is widely used in whole-exome sequencing and targeted region sequencing, and has great application value in clinical diagnosis, drug development, agricultural breeding and other fields. The service supports customization of sequences and concentrations. Capture probes range from 50–130 nt inlength, with exclusive HPLC purification, and are produced in a clean area to reduce exogenous contamination. The service features accurate quantitative aliquoting, stable modification, and extremely low base error rate.

Figure 1 Characteristics and application scenarios of hybridization capture probes

2. Customized Design Content

(1) Targeted Region Customization: Customizable combinations ranging from single genes and genefamilies to whole exomes.

(2) Difficult-to-Capture Region Optimization: Special design strategies for extremely high/low GC content regions, repetitive sequences, and homologous gene regions.

(3) Hotspot Mutation Compatibility: Improved capture efficiency and detection sensitivity for known hotspot mutation regions.

(4) Cross-Species Design: Supports diverse research needs for model and non-model organisms.

(5) Variant Type Adaptation: Optimized probe design for different variant types including SNV, Indel, CNV, and fusion genes.

3. Synthesis Advantages

(1) Full Customization: Designed entirely on demand, without limitations of standard panels.

(2) Algorithm Optimization: AI-assisted design algorithms to precisely balance specificity and coverage.

(3) Experimental Validation: Optional validation services to ensure performance in real samples.

(4) Cost-Effectiveness: Eliminates redundant sequences in commercial panels, reducing sequencing costs.

(5) Continuous Iteration: Probe design optimization based on result feedback for ongoing performance improvement.

4. Application Scenarios

(1) Rare Disease Research: High-sensitivity detection of ultra-low-frequency pathogenic variants.

(2) Cancer Precision Medicine: Clinical detection of gene mutations related to targeted therapy.

(3) Species-Specific Research: Gene structure and function analysis of non-model organisms.

(4) Complex Gene Regions: Accurate analysis of structurally complex or highly homologous regions.

(5) Multi-Omics Integration: Combined design compatible with both DNA and RNA capture.

5. Service Deliverables

(1) Complete Probe Sequences: All optimized probe sequences.

(2) Design Report: Including design principles, coverage analysis, specificity evaluation, etc.

(3) Sequencing Strategy Recommendations: Optimal sequencing scheme based on probe characteristics.

(4) Data Analysis Template: Recommended analysis pipeline optimized for probe design.

GeneRulor customized probe design service frees your research from the limitations of commercial panels and achievestruly on-demand customized precise capture, providing strong technical support for your scientific exploration and clinical translation!