GeneRulor pA-Transposase

1. Product Overview

GeneRulor pA-Transposase is a highly efficient transposase optimally designed through protein engineering, fused with the Protein A domain to endow it with the function of specifically binding to the Fc fragment of antibodies. This novel transposase not only retains the DNA cleavage and ligation activities of transposase but also achieves precise localization of specific chromatin target regions by virtue of the high-affinity binding between Protein A and antibodies. As a core component of the CUT&Tag technology, pA-Transposase can guide the transposase to be accurately enriched at specific genomic loci by binding to the specific primary antibody of the target protein, realizing in situ DNA fragmentation and sequencing adapter labeling, and thus serving as an ideal tool for high-resolution analysis of epigenetic modifications and transcription factor binding sites.

Compared with traditional ChIP-seq, the CUT&Tag technology based on pA-Transposase features distinct advantages such as low sample requirement, high signal-to-noise ratio and simple operation. Its core Protein A domain has universal and potent binding affinity for IgG antibodies (especially the Fc region of IgG) from multiple species including humans and rabbits, ensuring high efficiency and stability in commonly used experimental systems. This makes the tool particularly suitable for epigenetic research centered on human or rabbit antibodies, such as chromatin analysis of histone modifications. For experiments mainly using mouse IgG1 antibodies, pG-Transposase with complementary binding profiles can be selected for combination, providing users with a flexible and precise solution.

2. Application

CUT&Tag Technology: pA-Transposase can bind to specific antibodies to achieve precise labeling of regions of interest and library construction. Its working principle is to guide the transposase to target sites through the recognition of specific chromatin marks or DNA-binding proteins by antibodies, realizing highly specific DNA fragmentation and adapter ligation. This technology is especially suitable for studying low-abundance transcription factor binding sites and specific histone modifications, which can significantly improve sequencing efficiency and data quality, reduce background noise, and provide a powerful tool for precise epigenomic analysis.

Figure 1. Experimental flow chart of pA-Transposase for CUT&Tag

References

[1] Kaya-Okur HS, Wu SJ, Codomo CA, Pledger ES, Bryson TD, Henikoff JG, Ahmad K, Henikoff S. CUT&Tag for efficient epigenomic profiling of small samples and single cells. Nat Commun. 2019 Apr 29;10(1):1930. doi: 10.1038/s41467-019-09982-5.

[2] Farzad N, Enninful A, Bao S, Zhang D, Deng Y, Fan R. Spatially resolved epigenome sequencing via Tn5 transposition and deterministic DNA barcoding in tissue. Nat Protoc. 2024 Nov;19(11):3389-3425. doi: 10.1038/s41596-024-01013-y.