GeneRulor HBV Capture Kit

1. Product Description

Hepatitis B Virus (HBV) infection poses a severe threat to human health. Approximately 2 billion people are infected globally, and about 1.2 million people die each year from hepatitis, liver cirrhosis, and liver cancer caused by HBV infection. In China, more than 80% of liver cancer patients are HBV-infected. HBV is mainly transmitted through contact with infected blood or body fluids, with most infections occurring via blood exposure. Current studies indicate that HBV integration is a key mechanism leading to carcinogenesis. However, commonly used clinical HBV detection methods only allow genotyping and quantification, and cannot provide critical information on HBV integration.

The GeneRulor HBV Capture Kit employs probe design based on the HBVdb genomic database, fully covering the full-length sequences of more than 4,162 subtypes across eight HBV genotypes(A, B, C, D, E, F, G, H). It enables one-step analysis of integration break points at the HBV DNA level and identification of free HBV, which is of great scientific significance for clarifying the mechanism of virus‑related tumor development and progression.

2. Applications

(1) Accurate Genotyping: Covers all known HBV genotypes.

(2) Viral Load Quantification: Viral load can be assessed by comparison with housekeeping genes.

(3) Integration Determination: Integration is confirmed if human-HBV fusion reads are detected.

(4) Integration Site Detection: Integration site information is obtained by aligning detected human-HBV sequences to the reference genome.

References

[1]Tian, R. et al. Risk stratification of cervical lesions using capture sequencing and machine learning method based on HPV and human integrated genomic profiles. Carcinogenesis 40, 1220-1228,doi:10.1093/carcin/bgz094 (2019).

2]Wu, C. et al. DeepHBV: a deep learning model to predict hepatitis B virus (HBV) integration sites. BMC Ecol Evol 21,138, doi:10.1186/s12862-021-01869-8 (2021).