MS2-p65-HSF1 Transcriptional Activation Helper Protein

Product Introduction

MS2-p65-HSF1 is a key auxiliary protein component within the Synergistic Activation Mediator (SAM) CRISPRa system, representing a core innovation of second-generation CRISPR activation technology. This fusion protein comprises three functional elements: the MS2 phage coat protein, the NF-κB p65 transcriptional activation domain, and the Heat Shock Factor 1 (HSF1) activation domain. The MS2 domain specifically recognizes and binds to MS2 aptamer sequences within RNA stem-loops, while p65 and HSF1 provide potent transcriptional activation capabilities.

In the SAM system, researchers have engineered the conventional sgRNA by inserting MS2 aptamer sequences into its stem-loop structure. When dCas9-VP64 binds to a target gene promoter, these modified sgRNAs simultaneously expose MS2 binding sites, enabling efficient recruitment of the MS2-p65-HSF1 fusion protein.