Beads Linked Tn5 (BLT) is an innovative library preparation technology with Tn5 transposase pre-immobilized on magnetic bead surfaces. By shifting the fragmentation reaction from the traditional liquid phase to a solid-phase interface, it fully addresses the core industry pain point of conventional Tn5 library preparation: fragment size is heavily dependent on DNA input quantity.

Core Technical Advantages

• Solid-phase interface reaction: Ultra-high local enzyme concentration for significantly improved transposition efficiency and specificity;

• Saturation reaction mechanism: Fixed Tn5 loading on bead surface ensures highly uniform library fragment size;

• Streamlined purification: Completed via magnetic separation, eliminating multiple rounds of bead purification and tube transfer;

• 72-minute library preparation workflow, 15 minutes shorter than standard Tn5 protocols;

• Supports a broad DNA input range of 1 ng–500 ng;

• Optimally compatible with degraded DNA (FFPE, cfDNA, ancient DNA, eDNA, etc.);

• Fully compatible with automated platforms.

Competitive Advantages

Compared with BLT products from Company N, SUTONG BLT delivers superior performance in the following aspects:

• Cross-species compatibility: Excellent performance across samples from human skeletal muscle tissue, human Caski cell line, human blood, mouse tumor, rhesus monkey, sheep, E. coli, and more;

• Higher library concentration, with fragments more concentrated in the 200–500 bp range;

• Superior overall performance across the full 1 ng–500 ng DNA input range, with a more prominent advantage at higher DNA inputs.

Human Cot-1 DNA is a high-purity repetitive sequence blocking agent extracted from human placental DNA, enriched with repetitive sequences of the Alu and Kpn families. It preferentially binds to repetitive sequences in the library during hybrid capture assays, effectively preventing non-specific hybridization.

Core Values

• Eliminates non-specific hybridization: Effectively blocks repetitive sequences that account for approximately 50% of the human genome, significantly reducing the off-target rate.

• Improves capture efficiency: Ensures probes precisely bind to target regions, enhancing on-target rate and sequencing depth.

• Optimizes data quality: Improves library uniformity and coverage, while greatly reducing sequencing costs.

Stringent Quality Control System

• Precise fragment size control: Main peak at 50–300 bp, perfectly matching the kinetic requirements of NGS hybrid capture.

• High purity guarantee: A260/280 ratio strictly controlled within the range of 1.7–2.0.

• Viral nucleic acid testing: Each batch undergoes testing for HBV, HCV, HIV, HTLV and other viruses.

• Inter-batch stability: Strict SOP (Standard Operating Procedure) and batch record management to ensure consistent product performance.

Verified Performance Data

GeneRulor Human Cot-1 DNA comprehensively outperforms similar domestic and international competitors (BPA, JRCB) in core indicators including sequencing depth (9,200–9,400+), capture efficiency (~50.8%), and uniformity (98.85–98.97). It has earned recognition from leading industry customers including Nanjing Geneseeq, BGI, Berry Genomics, Amoy Diagnostics, Jiangsu Honglin, LBP Medicine, InVitae Bio, and DyneGen.

This superparamagnetic bioseparation system is developed based on the ultra-high affinity of the streptavidin-biotin interaction (Kd ≈ 10⁻¹⁴ mol/L). High-density streptavidin modification delivers exceptional binding capacity, while the optimized surface blocking process ensures extremely low non-specific binding (NSB).

Two Specifications Available

• SA Magnetic Beads （1μm）: High specific surface area, with ssDNA binding capacity > 500 pmol/mg and dsDNA binding capacity > 20 μg/mg. Ideal for hybrid capture of trace samples, single-cell sequencing library preparation, and high-sensitivity molecular diagnostics.

• SA Magnetic Beads （3μm）: Faster magnetic response and lower non-specific binding, with ssDNA binding capacity > 200 pmol/mg and dsDNA binding capacity > 10 μg/mg. Suitable for high-throughput batch sample processing on automated platforms, protein purification, and immunoprecipitation assays.

Competitive Advantages

1. The ssDNA binding capacity of 1μm beads is over 25% higher than imported products from Competitors.

2. 3μm beads deliver over 27% higher ssDNA binding capacity and over 22% higher dsDNA binding capacity than products from Competitors.

3. Comprehensively outperforms imported magnetic beads in capture product concentration, efficiency, sequencing depth, and uniformity.

4. Stable performance in accelerated aging tests (10 consecutive weeks at 25°C/37°C, simulating 2 years of storage at 4°C).

Application Scenarios

• NGS Hybrid Capture

• Protein Purification

• Immunoprecipitation and Interaction Studies