Yeast System Protein Expression and Purification

The yeast expression system is the most cost-effective eukaryotic system for producing secreted and intracellular proteins. It combines the advantages of both prokaryotic and eukaryotic expression systems:

✓ High expression levels ✓ Low cost ✓ Easy scalability ✓ Proper protein folding

This makes it an ideal choice for applications ranging from small-scale experiments to industrial production.

GeneRulor offers gene expression services based on yeast expression systems, delivering high-quality protein products that meet client expectations with industry-leading cost-effectiveness, exceptional quality, and professional, efficient technical support.

2、Core Technology Advantages

I. Advanced Yeast Expression Platform

Utilizes a dual-system approach with Pichia pastoris and Saccharomyces cerevisiae.

Pichia pastoris System:

(1) Potent methanol-inducible promoter (AOX1)

(2) Ultra-high-density fermentation (wet cell weight >10 g/L)

(3) Superior secretion efficiency (5-10 g/L)

Saccharomyces cerevisiae System:

(1) Well-established genetic manipulation toolbox

(2) Native protein glycosylation pathway

(3) Generally Recognized as Safe (GRAS) expression host

II. Advantages of Eukaryotic Expression:

(1) Accurate protein folding

(2) Post-translational modifications (glycosylation/phosphorylation)

(3) Secretory expression efficiency reaching 5-10 g/L

III. Multi-Step Chromatography Technology:

(1) Affinity chromatography (His/Strep-tag)

(2) Ion exchange chromatography (IEX)

(3) Size exclusion chromatography (SEC)

IV. Stringent Quality Control Standards:

(1) >95% purity (verified by SDS-PAGE)

(2) Endotoxin-free

3、End-to-End Service

One-Stop Production from Gene to Protein

Includes:

(1) Gene optimization (2–3 weeks)

(2) Codon optimization

(3) Vector construction

(4) Strain development

(5) High-efficiency transfection technology

(6) High-throughput expression screening

(7) Fermentation scale-up

(8) Purification and quality control

(9) Lyophilization and formulation

Standard Delivery Timeline: 6–8 weeks