GeneRulor pA/G-Transposome

1. Product Overview

GeneRulor pA/G-Transposome combines all the advantages of GeneRulor pA-Transposome and GeneRulor pG-Transposome, offering a   superior antibody binding range and experimental flexibility. GeneRulor pA/G-Transposome can efficiently bind to almost all types of IgG antibodies, including those from multiple sources such as rabbit, mouse, sheep, cattle, human and their various subtypes, and exhibits excellent affinity especially for mouse IgG1, which is difficult to bind to. As a ready-to-use reagent, GeneRulor pA/G-Transposome eliminates the need for users to perform self-assembly steps, significantly simplifies the experimental procedures of CUT&Tag and CUT&RUN, and provides a solution for epigenetic research.

2. Product Features

(1) Ultra-broad antibody compatibility: Binds to almost all IgG subtypes, including the hard-to-bind mouse IgG1;

(2) Ready-to-use design: Pre-loaded with adapters, ready to use upon unboxing without additional assembly steps;

(3) High sensitivity: Capable of processing ultra-trace samples with as few as 500 cells;

(4) Versatile applications: Suitable for CUT&Tag, CUT&RUN and other epigenetic analysis technologies;

(5) High reproducibility: Excellent batch-to-batch stability ensures reliable and consistent experimental results.

3. Applications

CUT&Tag Technology: pA/G-Transposome can bind to specific antibodies to achieve accurate labeling and library construction of regions of interest. Its working principle is to guide transposase to target sites through the recognition of specific chromatin marks or DNA-binding proteins by antibodies, realizing highly specific DNA fragmentation and adapter ligation. This technology is particularly suitable for studying low-abundance transcription factor binding sites and specific histone modifications, which can significantly improve sequencing efficiency and data quality, reduce background noise, and provide a powerful tool for precise epigenomic analysis.

Figure 1. Experimental flow chart of CUT&Tag using pA/G-Transposase

References

[1] Kaya-Okur HS, Wu SJ, Codomo CA, Pledger ES, Bryson TD, Henikoff JG, Ahmad K, Henikoff S. CUT&Tag for efficient epigenomic profiling of small samples and single cells. Nat Commun. 2019 Apr 29;10(1):1930. doi: 10.1038/s41467-019-09982-5.

[2] Farzad N, Enninful A, Bao S, Zhang D, Deng Y, Fan R. Spatially resolved epigenome sequencing via Tn5 transposition and deterministic DNA barcoding in tissue. Nat Protoc. 2024 Nov;19(11):3389-3425. doi: 10.1038/s41596-024-01013-y.