GeneRulor Transposome

1. Product Overview

GeneRulor Transposome is a pre-assembled transposome complex, whose core components include the highly active GeneRulor Transposase obtained by protein engineering-directed evolution and specific DNA adaptors pre-embedded in the enzyme. Derived from Escherichia coli, this transposase has a significantly enhanced activity compared with the native enzyme, with a markedly improved transposition efficiency, exhibiting ultra-high transposition efficiency.

This complex can highly specifically recognize DNA fragments with Mosaic End (ME) sequences at both ends. Its unique mechanism of action lies in its ability to randomly bind to target DNA in a single reaction and simultaneously complete DNA cleavage and the insertion of the carried DNA fragments. This one-step tagging process that combines DNA fragmentation and adaptor ligation into a single step is known as the Tagmentation reaction. The technology exhibits extremely low sequence preference for DNA samples with different GC contents, ensuring broad applicability. For this reason, GeneRulor Transposome is particularly suitable for the construction of high-throughput sequencing libraries, which can greatly simplify the experimental workflows of applications such as whole-genome sequencing and ATAC-seq, not only saving operation time but also reducing experimental costs.

2. Product Features

(1) Pre-assembled: Adaptors are pre-embedded in the transposase, ready for immediate use, simplifying the experimental workflow;

(2) Ultra-high activity: Optimized by protein engineering, the transposition efficiency is 5-10 times higher than that of the native enzyme;

(3) Broad substrate compatibility: Extremely low sequence preference for DNA samples with different GC contents (20%-80%);

(4) High specificity: Precisely recognizes ME sequences to minimize non-specific reactions;

(5) Batch-to-batch consistency: A strict quality control system ensures stable performance across batches.

3. Applications

Utilizing its special transposition mechanism, GeneRulor Transposome can simultaneously complete two steps of DNA fragmentation and adaptor ligation in a single reaction. This one-step tagging method greatly streamlines the experimental workflow, not only saving operation time but also reducing experimental costs, making the library construction process more efficient.

It is applicable for: (1) Whole-genome sequencing library construction; (2) ATAC-seq library construction.

Figure 1. Schematic diagram of the workflow for second-generation sequencing library construction

References

[1] Li N, Jin K, Bai Y, Fu H, Liu L, Liu B. Tn5 Transposase Applied in Genomics Research. Int J Mol Sci. 2020 Nov 6;21(21):8329. doi: 10.3390/ijms21218329.

[2] Zhang H, Lu T, Liu S, Yang J, Sun G, Cheng T, Xu J, Chen F, Yen K. Comprehensive understanding of Tn5 insertion preference improves transcription regulatory element identification. NAR Genom Bioinform. 2021 Oct 27;3(4):lqab094. doi: 10.1093/nargab/lqab094.