GeneRulor ATAC-seq Library Prep Kit for Illumina

1. Product Overview

GeneRulor ATAC-seq Library Prep Kit for Illumina is a library construction kit developed for the Illumina high-throughput sequencing platform, dedicated to the study of chromatin openness/accessibility. Its principle is to use Tn5 transposase to cleave open chromatin regions at a specific time and space, and then analyze the sequencing tags introduced by Tn5 through high-throughput sequencing technology to obtain genome-wide information on open chromatin regions under that state. This kit is especially suitable for epigenetic research fields such as cancer research, developmental biology and aging. It can rapidly and accurately reflect the epigenetic status of chromatin, helping researchers to deeply explore the mechanisms of gene regulation.

2. Experimental Principle

This kit uses Tn5 transposase for targeted cleavage of open chromatin regions and direct insertion of sequencing adaptors at the cleavage sites. A sequencing library suitable for the Illumina platform is then constructed through PCR amplification and purification steps, enabling efficient analysis of genome-wide chromatin accessibility.

Figure 1. Schematic diagram of the ATAC-seq experiment

3. Applications

(1) Developmental biology and cell differentiation

Tracking the dynamics of chromatin opening during embryonic/tissue development and revealing the activation sequence of key regulatory elements.

(2) Cancer and tumor epigenetics

Comparing chromatin differences between tumor and normal tissues, and identifying tumor-specific enhancers and potential drug targets.

(3) Immunology and immune cell function

Analyzing the regulatory landscape of T/B cells, macrophages and other immune cells under activated/diseased states to study the mechanisms of immune response.

(4) Plant and agricultural biology

Deciphering chromatin changes in plant growth, stress resistance and metabolic regulation to support the research on agronomic trait improvement.

(5) Single-cell level (scATAC-seq / snATAC-seq)

Resolving the regulatory networks of tissue heterogeneity and rare cell subpopulations, often combined with single-cell RNA-seq to construct gene regulatory networks (GRN).

(6) Spatial epigenetics (spatial ATAC-seq)

Preserving spatial positions in tissue sections while analyzing chromatin openness to construct spatiotemporal regulatory maps.

(7) Other interdisciplinary applications

Epigenetic mechanism research in fields such as neurodegenerative diseases, evolutionary comparison, drug screening, and microbe-host interactions.