3 μm Streptavidin Magnetic Beads

1. Product Advantages

(1) Affinity binding: GR 3 μm SA Magnetic Beads have extremely high biotin-binding affinity, ensuring high capture efficiency.

(2) High purity and low non specific binding: The surface of the magnetic beads is uniformly coated with streptavidin, which reduces non-specific binding and improves the accuracy of experiments.

(3) Rapid separation capability: The magnetic properties enable rapid separation of target molecules, significantly reducing experimental time.

(4) High stability: Minimal batch-to-batch variation, suitable for avariety of experimental requirements.

(5) High cost performance: International quality at domestic prices.

2. Quality Control Results

Compared with imported SA magnetic beads, GR 3 μm SA Magnetic Beads of different batches show superior binding capacity to single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA).

Figure 1. Excellent ssDNA binding capacity of 3 μm SA Magnetic Beads from different batches

Figure 2. Excellent dsDNA binding capacity of 3 μm SA Magnetic Beads from different batches

3. Stability Test Results

In the accelerated aging test, the core basis for the correlation between temperature and time is the Q10 rule of the Arrhenius Equation, which establishes a quantitative relationship between temperature and degradationrate through chemical reaction kinetics theory. It is defined as: the multipleby which the chemical reaction rate increases for every 10 °C rise in temperature (Q10 is usually 2~4, and Q10=2 is commonly adopted for biological products).

Temperature-time conversion: Acceleration factor = 2^((Accelerated temperature - Actual temperature)/10)

4 °C → 25 °C (temperature difference of 21 °C): 1 month at 25 °C ≈4.3 months at 4 °C

4 °C → 37 °C (temperature difference of 33 °C): 1 month at 37 °C ≈9.8 months at 4 °C

Figure 3. Excellent ssDNA binding capacity of 3 μm SA Magnetic Beads in stability test

Figure 4. Excellent dsDNA binding capacity of 3 μm SA Magnetic Beads in stability test

4. Capture Concentration

GR 3 μm SA Magnetic Beads are particularly suitable for liquid phase hybridization capture. Under the same magnetic bead dosage and identical PCR cycle parameters, GR 3 μm SA Magnetic Beads of different batches yield better Post-PCR product concentrations compared with imported SA magnetic beads.

Figure 5. Superior capture concentration of 3 μm SA Magnetic Beads from different batches compared with imported magnetic beads

5. Capture Efficiency

Capture efficiency refers to the percentage of sequencing data mapped to the target regions. The higher the capture efficiency, the greater the probability that some important target regions can be captured. Magnetic beads play a key role in liquid phase hybridization capture, and their characteristics directly affect the capture efficiency. In the comparison of off-machine data, GR 3 μm SA Magnetic Beads of all different batches exhibit slightly better capture efficiency than the imported magnetic beads from Company T.

Figure 6. Slightly better capture efficiency of 3 μm SA Magnetic Beads from different batches compared with imported magnetic beads

6. Capture Coverage

Capture coverage represents the proportion of successfully captured target sequences in all regions covered by the designed probes, which should ideally be close to 100%. In comparative experiments at different sequencing depths, the capture coverage of GR 3 μm SA Magnetic Beads is on par with that of the imported magnetic beads from Company T, both showing high capture coverage.

Figure 7. Capture coverage of 3 μm SA Magnetic Beads from different batches is equivalent to that of imported magnetic beads

7. Comparison of Capture Uniformity

When evaluating the sequencing coverage of target regions, capture uniformity is an important indicator for measuring sequencing quality. It reflects the consistency of the sequencing depth distribution across different target regions. The higher the uniformity, the smaller the difference insequencing depth among various regions, and the more stable and reliable the capture efficiency. Experimental comparison shows that GR 3 μm SA Magnetic Beads exhibit excellent performance comparable to the imported magnetic beads from Company T, both reaching a high level of capture uniformity.

Figure 8. Capture uniformity of 3 μm SA Magnetic Beads from different batches is equivalent to that of imported magnetic beads