Product

Item No.

Product Name

Replicon

for Host

Strain

Replicon for E.coli

Selection

Marker

Promoter

Inducible System

Cas Protein

Fluorescent

Protein

pST001

RepEDG-D10A-luxS-sgRNA1-traJ

pAMβ1

Ori

Kan

PbacA

TetR

nCas9(D10A)

EGFP

pST002

RepEDG-D10A-luxS-sgRNA2-traJ

pAMβ1

Ori

Kan

PbacA

TetR

nCas9(D10A)

EGFP

pST003

RepEDG-D10A-luxS-sgRNA3-traJ

pAMβ1

Ori

Kan

PbacA

TetR

nCas9(D10A)

EGFP

pST004

pJOE8999-luxS

pE194ts

Ori

Erm,   Crm/Cam

PbacA

pST005

pB025-FnCas12a-sfGFP-eno-CmRdonor-3-51

repB

R6k

ErmG,   Crm/Cam

P1

TetR

FnCas12a

sfGFP

pST006

pB025-FnCas12a-sfGFP-eno-CmRdonor-2-65

repB

R6k

ErmG,   Crm/Cam

P1

TetR

FnCas12a

sfGFP

pST007

pB025-FnCas12a-sfGFP-eno-CmRdonor-1-33

repB

R6k

ErmG,   Crm/Cam

P1

TetR

FnCas12a

sfGFP

pST008

RepEDG-Kan-Erm-TetR-D10A-oriT-tuf

pAMβ1

Ori

Erm,   Kan

EGFP

pST009

pAMβ1-Kan-Erm-Tet-xylose-D10A-

nisinZ-AtoC-sgRNA

pAMβ1

Ori

Erm,   Kan, Tet/TcR

xylose

xylR

pST010

pBBR1-Cas9-P43-Fur   gRNA1-BB Fur HA-ErmR

repB

pBBR1

ErmG,   Amp

P43

IPTG

SpCas9

pST011

pUT-ara-Cas9-P43-Fur gRNA1-BB Fur HA-ErmR

R6k

ErmG

araBAD

pST012

pFC332-sgRNA

AMA1

Ori

Hyg

TEF，gpdA，trpC

pST013

pHSE401-AhU6-DsRed

pVS1 oriV

Kan, SmR

CaMV 35S

SpCas9

pST014

M13-Cas9

CloDF13 ori

Crm/Cam

araBAD

SpCas9

mcherry

pST015

pACRISPR-mScarlet3-kana

pRO1600 oriV

Ori, pRO1600 oriV

Kan

mSarlet

pST016

pACRISPR-DsRed2-kana

pRO1600 oriV

Ori, pRO1600 oriV

Kan

DsRed2

pST017

pACRISPR-DsRed2-AmpR

pRO1600 oriV

Ori, pRO1600 oriV

Amp

DsRed2

pST018

pnCasPA-BEC-TEM-1

pRO1600 oriV

Ori, pRO1600 oriV

GmR

CBE(BE3)

pST019

pnCasPA-BEC-TEM-2

pRO1600 oriV

Ori, pRO1600 oriV

GmR

CBE(BE3)

pST020

pACRISPR

pRO1600 oriV

Ori, pRO1600 oriV

Amp

pST021

pCasPA

oriV

oriV

Tet/TcR

SpCas9

pST022

pJOE8999

pE194ts

Ori

Kan

Pman

SpCas9

pST023

pLCNICK

pAMβ1

pSC101 ori-RepA101(Ts)

Kan

P23，Pldh

SpCas9

pST024

pWSCas9n-araBAD-A1-mcherry-

1246gp41-sfGFP-kan-sgRNA89

pE194ts

Ori

Kan

P43

araBAD

CBE(Plug-in BE3)

pST025

pMGCas9n-gatAX-Erm-sgRNA-1

pWV01

Ori

Erm, Kan

P23，Pldh

nCas9(D10A)

pST026

pMGCas9n-gatAX-Erm-sgRNA-2

pWV01

Ori

Erm, Kan

P23，Pldh

nCas9(D10A)

pST027

pMTLCas9-Erm

repA

Ori

ErmG, Crm/Cam

Pfdx

SpCas9

pST028

pMTL-gRNA-AcnA

repA

Ori

Crm/Cam

Psyn

pST029

pACRISPR-mcherry-kana

pRO1600 oriV

Ori

Kan

mcherry

pST030

pUC57-crRNA-info-cat-kan-sfGFP

Ori

Amp

sfGFP

pST031

pACRISPR-yjcS

pRO1600 oriV

Ori

Amp

pST032

pWSCas9n-BEC-A56

pE194ts

Ori

Kan

nCas9(D10A)

pST033

pWSCas9n-sgRNA-A

pE194ts

Ori

Kan

pST034

pCas9n-BEC-qnrA-asnB

pRO1600 oriV

Ori

GmR

pST035

pHel3-Cas9n-BEC-CagA

Ori

Kan

pST036

araBAD-A1-mcherry-1246gp41

-sfGFP-gcvT-sgRNA

pRO1600 oriV

Ori

GmR

pST037

pABEl-mCherry-Cas9n-sfGFP-Fn

Ori

Amp

pST038

pACRISPR-123kb

pRO1600 oriV

Ori

Amp

pST039

YT1-Cox

pE194ts

Ori

Kan, Crm/Cam

pST040

RepEDG-Amp-Erm-prgB-EGFP

-prgC-2sgRNA

pAMβ1

Ori

Erm, Amp

PbacA

EGFP

pST041

RepEDG-Kan-Erm-prgB-EGFP-

prgC-2sgRNA-oriT-TetR-D10A

pAMβ1

Ori

Erm, Kan

PbacA

TetR

nCas9(D10A)

EGFP

pST042

RepEDG-Kan-Erm-TetR-

1246gp41-BE3-oriT-ldhAsgRNA3

pAMβ1

Ori

Erm, Kan

PbacA

TetR

CBE(Plug-in BE3)

pST043

RepEDG-Kan-Erm-TetR-

AsCas12a-2xcrRNA-KOdonor

pAMβ1

Ori

Erm, Kan

PbacA

TetR

AsCas12a

pST044

RepEDG-Kan-Erm-TetR-

BE3-oriT-ldhAsgRNA3

pAMβ1

Ori

Erm, Kan

PbacA

TetR

CBE(BE3)

pST045

RepEDG-Kan-Erm-TetR-crRNA

-citE-KOdonor

pAMβ1

Ori

Erm, Kan

PbacA

TetR

pST046

RepEDG-Kan-Erm-TetR-D10A

-citE-KOdonor-sgRNA1

pAMβ1

Ori

Erm, Kan

PbacA

TetR

nCas9(D10A)

pST047

RepEDG-Kan-Erm-TetR-D10A

-evoCDA1-oriT-ldhAsgRNA3

pAMβ1

Ori

Erm, Kan

PbacA

TetR

CBE(TargetAID)

pST048

RepEDG-Kan-Erm-TetR-D10A

-hutH-KOdonor-sgRNA1

pAMβ1

Ori

Erm, Kan

PbacA

TetR

nCas9(D10A)

pST049

RepEDG-Kan-Erm-TetR-D10A

-hutH-KOdonor-sgRNA2

pAMβ1

Ori

Erm, Kan

PbacA

TetR

nCas9(D10A)

pST050

RepEDG-Kan-Erm-TetR-D10A

-KOdonor-AW-ldhAsgRNA355

pAMβ1

Ori

Erm, Kan

PbacA

TetR

nCas9(D10A)

pST051

RepEDG-Kan-Erm-TetR-D10A

-KOdonor-oriT-AW-ldhAsgRNA355

pAMβ1

Ori

Erm, Kan

PbacA

TetR

nCas9(D10A)

pST052

RepEDG-Kan-Erm-TetR-D10A-mCherry

donor-oriT-AW-ldhAsgRNA355

pAMβ1

Ori

Erm, Kan

PbacA

TetR

nCas9(D10A)

EGFP, mcherry

pST053

RepEDG-Kan-Erm-TetR-D10A-mCherry

Ermdonor-oriT-AW-ldhAsgRNA355

pAMβ1

Ori

Erm, Kan

PbacA

TetR

nCas9(D10A)

EGFP, mcherry

pST054

RepEDG-Kan-Erm-TetR-FrCas9-

KOdonor-oriT-ldhAsgRNA3

pAMβ1

Ori

Erm, Kan

PbacA

TetR

FrCas9

pST055

RepEDG-Kan-Erm-TetR-SpCas9-

KOdonor-oriT-AW-ldhAsgRNA355

pAMβ1

Ori

Erm, Kan

PbacA

TetR

SpCas9

pST056

RepEDG-Kan-Erm-TetR-SpCas9

-KOdonor-AW-ldhAsgRNA355

pAMβ1

Ori

Erm, Kan

PbacA

TetR

SpCas9

pST057

pMG-P23-Cas9-sfGFP-

gatAXsgRNA-Ermdonor-4

pWV01

Ori

Erm, Kan

P23，Pldh

SpCas9

sfGFP

pST058

pMG-PbacA-D10A-EGFP-

gatAXsgRNA-Ermdonor

pWV01

Ori

Erm, Kan

P23，Pldh

nCas9(D10A)

EGFP

pST059

pMG-PbacA-FrCas9-732-1103

-EGFP-gatAXsgRNA-Ermdonor

pWV01

Ori

Erm, Kan

P23，Pldh

FrCas9

EGFP

pST060

pMG-PbacA-SpCas9-EGFP-

gatAXsgRNA-Ermdonor

pWV01

Ori

Erm, Kan

P23，Pldh

SpCas9

EGFP

pST061

pMG36e-Crm-Erm-Amp-prgB-EGFP

-prgC-2sgRNA-oriT-TetR-D10A

pWV01

Ori

Erm, Amp, Crm/Cam

PbacA

TetR

nCas9(D10A)

EGFP

pST062

pMG36e-Crm-Erm-Amp-prgB-EGFP

-prgC-2sgRNA-oriT-TetR-SpCas9

pWV01

Ori

Erm, Amp, Crm, Erm, Amp, Crm/Cam

PbacA

TetR

SpCas9

EGFP

pST063

pMG36e-Crm-Erm-P32-PbacA

-D10A-EGFP-oriT

pWV01

Ori

Erm, Crm/Cam

PbacA，P32

TetR

nCas9(D10A)

EGFP

pST064

pMG36e-Crm-Erm-P32-PbacA

-D10A-EGFP

pWV01

Ori

Erm, Crm/Cam

PbacA，P32

TetR

nCas9(D10A)

EGFP

pST065

pMG36e-Crm-Erm-TetR-D10A-EGFP

-oriT-KOdonor-AW-ldhAsgRNA355

pWV01

Ori

Erm, Crm/Cam

TetR

nCas9(D10A)

EGFP

pST066

pMG36e-Crm-Erm-TetR-SpCas9-EGFP

-oriT-KOdonor-AW-ldhAsgRNA355

pWV01

Ori

Erm, Crm/Cam

TetR

SpCas9

EGFP

pST067

pMG36e-Crm-PbacA-D10A-sfGFP

pWV01

Ori

Crm/Cam

PbacA

nCas9(D10A)

sfGFP

pST068

pMG36e-Crm-PbacA-SpCas9-sfGFP

pWV01

Ori

Crm/Cam

PbacA

SpCas9

sfGFP

pST069

pMG36e-Kan-Erm-Pbaca-SpCas9-EGFP

pWV01

Ori

Erm, Kan

PbacA

SpCas9

EGFP

pST070

pMG36e-Kan-Erm-Pbaca-SpCas9-EGFP-oriT

pWV01

Ori

Erm, Kan

PbacA

SpCas9

EGFP

pST071

pMG36e-Kan-Erm-Pbaca-SpCas9-sfGFP-oriT

pWV01

Ori

Erm, Kan

PbacA

SpCas9

sfGFP

pST072

pMG36e-kana-SpCas9-GSlinker-Erm

pWV01

Ori

Erm, Kan

Pldh

SpCas9

pST073

araBAD-1246gp41-BE3-amp-sgRNA1

pRO1600 oriV

Ori, pRO1600 oriV

GmR

trc

araBAD

CBE(Plug-in BE3)

pST074

araBAD-1246gp41-BE3-amp-sgRNA2

pRO1600 oriV

Ori, pRO1600 oriV

GmR

trc

araBAD

CBE(Plug-in BE3)

pST075

araBAD-1246gp41-BE3-amp-sgRNA3

pRO1600 oriV

Ori, pRO1600 oriV

GmR

trc

araBAD

CBE(Plug-in BE3)

pST076

araBAD-1246gp41-BE3-amp-sgRNA4

pRO1600 oriV

Ori, pRO1600 oriV

GmR

trc

araBAD

CBE(Plug-in BE3)

pST077

araBAD-1246gp41-CBEmax-amp-sgRNA1

pRO1600 oriV

Ori, pRO1600 oriV

GmR

trc

araBAD

CBE(Plug-in TargetAID)

pST078

araBAD-1246gp41-CBEmax-amp-sgRNA2

pRO1600 oriV

Ori, pRO1600 oriV

GmR

trc

araBAD

CBE(Plug-in TargetAID)

pST079

araBAD-A1-mcherry-1246gp41-sfGFP-

amp-sgRNA1

pRO1600 oriV

Ori, pRO1600 oriV

GmR

trc

araBAD

CBE(Plug-in BE3)

sfGFP, mcherry

pST080

araBAD-A1-mcherry-1246gp41-sfGFP-

amp-sgRNA2

pRO1600 oriV

Ori, pRO1600 oriV

GmR

trc

araBAD

CBE(Plug-in BE3)

sfGFP, mcherry

pST081

araBAD-A1-sfGFP-amp-sgRNA2

pRO1600 oriV

Ori, pRO1600 oriV

GmR

trc

araBAD

CBE(Plug-in BE3)

sfGFP

pST082

araBAD-AID-mcherry-1246gp41-

sfGFP-amp-sgRNA1

pRO1600 oriV

Ori, pRO1600 oriV

GmR

trc

araBAD

CBE(Plug-in TargetAID)

sfGFP, mcherry

pST083

araBAD-AID-mcherry-1246gp41-

sfGFP-amp-sgRNA2

pRO1600 oriV

Ori, pRO1600 oriV

GmR

trc

araBAD

CBE(Plug-in TargetAID)

sfGFP, mcherry

pST084

araBAD-BE3-amp-sgRNA1

pRO1600 oriV

Ori, pRO1600 oriV

GmR

trc

araBAD

CBE(BE3)

pST085

araBAD-BE3-amp-sgRNA2

pRO1600 oriV

Ori, pRO1600 oriV

GmR

trc

araBAD

CBE(BE3)

pST086

araBAD-BE3-Tet-GOBsgRNA1

oriV

oriV

Tet/TcR

Pldh

araBAD

CBE(BE3)

pST087

araBAD-BE3-Tet-GOBsgRNA2

oriV

oriV

Tet/TcR

Pldh

araBAD

CBE(BE3)

pST088

araBAD-BE3-Tet-GOBsgRNA3

oriV

oriV

Tet/TcR

Pldh

araBAD

CBE(BE3)

pST089

araBAD-D10A-evoCDA1-amp

-sgRNA1

pRO1600 oriV

Ori, pRO1600 oriV

GmR

trc

araBAD

CBE(TargetAID)

pST090

pACRISPR-sgRNA-pheA-up

-5.4k-dn

pRO1600 oriV

Ori, pRO1600 oriV

Amp

trc

araBAD

pST091

pB025-FnCas12a-sfGFP-43858

-Ampdonor

repB

R6k

ErmG, Amp

P1

TetR

FnCas12a

sfGFP

pST092

pB025-FnCas12a-sfGFP-43858

Kana-donor

repB

R6k

ErmG, Amp, Kan

P1

TetR

FnCas12a

sfGFP

pST093

pB025-FnCas12a-sfGFP-GOB-crRNA1

repB

R6k

ErmG, Amp

P1

TetR

FnCas12a

sfGFP

pST094

pB025-FnCas12a-sfGFP-GOB-crRNA2

repB

R6k

ErmG, Amp

P1

TetR

FnCas12a

sfGFP

pST095

pB025-FnCas12a-sfGFP-GOB-crRNA3

repB

R6k

ErmG, Amp

P1

TetR

FnCas12a

sfGFP

pST096

pB025-tetR-D10A-EGFP-GOB-KOdonor

-sgRNA1

repB

Ori

Kan, ErmG

trc,PbacA

TetR

nCas9(D10A)

EGFP

pST097

pB025-tetR-D10A-EGFP-GOB-KOdonor

-sgRNA2

repB

Ori

Kan, ErmG

trc,PbacA

TetR

nCas9(D10A)

EGFP

pST098

pJC005.em

pAMβ1

Ori

Erm

TetR

AsCas12a

pST099

RepEDG-Kan-Erm-TetR-dSpCas9

-evoCDA1-oriT-citEsgRNA1

pAMβ1

Ori

Erm, Kan

TetR

CBE(TargetAID)

pST100

RepEDG-Kan-Erm-TetR-D10A

-citE-KOdonor-sgRNA4

pAMβ1

Ori

Erm, Kan

TetR

nCas9(D10A)

pST101

RepEDG-Kan-Erm-TetR-D10A

-citE-KOdonor-sgRNA3

pAMβ1

Ori

Erm, Kan

TetR

nCas9(D10A)

pST102

RepEDG-Kan-Erm-TetR-D10A

-citE-KOdonor-sgRNA2

pAMβ1

Ori

Erm, Kan

TetR

nCas9(D10A)

pST103

RepEDG-Kan-Erm-TetR-D10A

-citE-KOdonor-oriT-sgRNA2

pAMβ1

Ori

Erm, Kan

TetR

nCas9(D10A)

pST104

RepEDG-Kan-Erm-TetR-D10A

-citE-KOdonor-oriT-sgRNA1

pAMβ1

Ori

Erm, Kan

TetR

nCas9(D10A)

pST105

OriV-Tet-GOB-2xsgRNA1

oriV

Tet/TcR

Pldh，PbacA

pST106

OriV-Tet-GOB-2xsgRNA2

oriV

Tet/TcR

Pldh，PbacA

pST107

pB025-T7-ChCas12f1-GOB-

KOdonor-sgRNA1

repB

Ori

ErmG, Crm/Cam

T5，J23119

IPTG

ChCas12f1

pST108

pB025-T7-ChCas12f1-GOB-

KOdonor-sgRNA2

repB

Ori

ErmG, Crm/Cam

T5，J23119

IPTG

ChCas12f1

pST109

pB025-λ-FnCas12a-sfGFP

-GOB-crRNA3

repB

R6k

ErmG, Amp

P1

TetR, araBAD

FnCas12a

sfGFP

pST110

pB025-λ-tetR-D10A-EGFP

-GOB-KOdonor-2xsgRNA1

repB

Ori

ErmG, Kan

Pldh，PbacA

TetR, araBAD

nCas9(D10A)

EGFP

pST111

pCasPA-GOB-KO-donor-

D10A-2xsgRNA1

oriV

Tet/TcR, Hyg

Pldh，PbacA

araBAD

nCas9(D10A)

pST112

pCasPA-GOB-KO-donor

-SpCas9-2xsgRNA1

oriV

Tet/TcR, Hyg

Pldh，PbacA

araBAD

SpCas9

pST113

pCasPA-TcR-GOB-2xsgRNA1

oriV

Tet/TcR, Hyg

Pldh，PbacA

araBAD

pST114

pCasPA-TcR-GOB-Erm-2xsgRNA1

oriV

Tet/TcR, Hyg, ErmG

Pldh，PbacA

araBAD

pST115

RepEDG-Kan-Erm-TetR-dead

   BE3-oriT-GOBsgRNA1

pAMβ1

Ori

Erm, Kan

TetR

CBE(BE3)

pST116

RepEDG-Kan-Erm-TetR-dead

   BE3-oriT-GOBsgRNA4

pAMβ1

Ori

Erm, Kan

TetR

CBE(BE3)

pST117

RepEDG-Kan-Erm-TetR-dead

   BE3-oriT-GOBsgRNA5

pAMβ1

Ori

Erm, Kan

TetR

CBE(BE3)

pST118

RepEDG-Kan-Erm-TetR-dSpCas9

-evoCDA1-oriT-GOBsgRNA2

pAMβ1

Ori

Erm, Kan

TetR

CBE(TargetAID)

pST119

RepEDG-Kan-Erm-TetR-dSpCas9

-evoCDA1-oriT-GOBsgRNA3

pAMβ1

Ori

Erm, Kan

TetR

CBE(TargetAID)

pST120

RepEDG-Kan-Erm-TetR-GoCas12m

-APOBEC1-oriT-GOBsgRNA1

pAMβ1

Ori

Erm, Kan

TetR

GoCas12m-BE3

pST121

RepEDG-Kan-Erm-TetR-GoCas12m

-evoCDA1-oriT-GOBsgRNA1

pAMβ1

Ori

Erm, Kan

TetR

GoCas12m-evoCDA1

pST122

RepEDG-Kan-Erm-TetR-MmCas12m

-APOBEC1-oriT-GOBsgRNA1

pAMβ1

Ori

Erm, Kan

TetR

MmCas12m-BE3

pST123

RepEDG-Kan-Erm-TetR-MmCas12m

-evoCDA1-oriT-GOBsgRNA1

pAMβ1

Ori

Erm, Kan

TetR

MmCas12m-evoCDA1

pST124

RepB-Kan-Erm-1PrtP

repB

Ori

ErmG, Kan

P32

EGFP

pST125

RepB-Kan-Erm-1PrtP-EGFP

repB

Ori

ErmG, Kan

P32

EGFP

pST126

RepB-Kan-Erm-2Cwh

repB

Ori

ErmG, Kan

P32

EGFP

pST127

RepB-Kan-Erm-2Cwh-EGFP

repB

Ori

ErmG, Kan

P32

EGFP

pST128

RepB-Kan-Erm-3Exp4

repB

Ori

ErmG, Kan

P32

EGFP

pST129

RepB-Kan-Erm-3Exp4-EGFP

repB

Ori

ErmG, Kan

P32

EGFP

pST130

RepB-Kan-Erm-4SlpA

repB

Ori

ErmG, Kan

P32

EGFP

pST131

RepB-Kan-Erm-4SlpA-EGFP

repB

Ori

ErmG, Kan

P32

EGFP

pST132

RepB-Kan-Erm-5USP45

repB

Ori

ErmG, Kan

P32

EGFP

pST133

RepB-Kan-Erm-5USP45-EGFP

repB

Ori

ErmG, Kan

P32

EGFP

pST134

RepEDG-Kan-Erm-1PrtP

pAMβ1

Ori

Erm, Kan

P32

EGFP

pST135

RepEDG-Kan-Erm-2Cwh

pAMβ1

Ori

Erm, Kan

P32

EGFP

pST136

RepEDG-Kan-Erm-3Exp4

pAMβ1

Ori

Erm, Kan

P32

EGFP

pST137

RepEDG-Kan-Erm-4SlpA

pAMβ1

Ori

Erm, Kan

P32

EGFP

pST138

RepEDG-Kan-Erm-5USP45

pAMβ1

Ori

Erm, Kan

P32

EGFP

pST139

RepEDG-Kan-Erm-TetR-D10A

-EGFP-KI-BLF-sgRNA1

pAMβ1

Ori

Erm, Kan

P32

TetR

nCas9(D10A)

EGFP

pST140

RepEDG-Kan-Erm-TetR-D10A

-EGFP-KI-BLF-sgRNA2

pAMβ1

Ori

Erm, Kan

P32

TetR

nCas9(D10A)

EGFP

pST141

RepEDG-TetR-EGFP-Kan-BLF-Erm

pAMβ1

Ori

Erm, Kan

P32

TetR

EGFP

pST142

pAMβ1-Kan-Erm-TetR-D10A-

fsrB-AtoT-sgRNA

pAMβ1

Ori

Erm, Kan

TetR

nCas9(D10A)

EGFP

pST143

pAMβ1-Kan-Erm-TetR-D10A-

fsrB-AtoT-sgRNA2

pAMβ1

Ori

Erm, Kan

TetR

nCas9(D10A)

EGFP

pST144

pAMβ1-Kan-Erm-Tet-xylose-

D10A-fsrB-AtoT-sgRNA

pAMβ1

Ori

Erm, Kan, Tet/TcR

xylose

nCas9(D10A)

EGFP

pST145

pLCNICK-prgB-EGFP-prgC-ldhsgRNA

pAMβ1

Ori

Amp

EGFP

pST146

pLCNICK-prgB-EGFP-prgC-1

pAMβ1

pSC101 ori-RepA101(Ts)

Erm, Kan

Pldh

EGFP

pST147

RepEDG-Amp-Erm-prgB-

EGFP-prgC-prgUsgRNA

pAMβ1

Ori

Amp, Erm

PbacA

EGFP

pST148

pLCNICK-prgB-EGFP-prgC

-prgUsgRNA

pAMβ1

Ori

Erm

EGFP

pST149

RepEDG-Kan-Erm-TetR-D10A-ldh-KI-

mCherry-oriT-AW-ldhAsgRNA355

pAMβ1

Ori

Erm, Kan

PbacA

TetR

nCas9(D10A)

mcherry

pST150

pAMβ1-Kan-Erm-TetR-D10A-oriT

-prgU-EGFP-sgRNA

pAMβ1

Ori

Erm, Kan

PbacA

TetR

nCas9(D10A)

EGFP

pST151

pUC57-gbcas9

Ori

Amp

gbCas9

pST152

pUC57-zpcas9

Ori

Amp

zpCas9

pST153

pet28a-zpCas9-tracrRNA+DR

Ori

Kan

J23119

IPTG

zpCas9

pST154

pet28a-FcCas9-tracrRNA+DR

Ori

Kan

J23119

IPTG

pST155

pet28a-frcas9√，又名pet28a

-FrCas9-PAM耗竭阳性对照

Ori

Kan

J23119

IPTG

FrCas9

pST156

pet28a-gbCas9-tracrRNA+DR

Ori

Kan

J23119

IPTG

gbCas9

pST157

6nt文库质粒图谱

p15A ori

Amp

pST158

pAMβ1-Kan-Erm-Tet-xylose

-D10A-fsrB-AtoT-sgRNA2

pAMβ1

Ori

Erm, Kan, Tet/TcR

xylose

nCas9(D10A)

EGFP

pST159

pUC19-crRNA1-ccdB

Ori

Amp

araBAD

pST160

pUC19-crRNA1-ccdB-B1-dcas12a

Ori

Amp

araBAD

Beacon1

pST161

pUC19-crRNA2-ccdB

Ori

Amp

araBAD

pST162

pUC19-sgRNA1-ccdB-Cas9

Ori

Amp

araBAD

SpCas9

pST163

pUC19-sgRNA2-ccdB

Ori

Amp

araBAD

pST164

pUC19-sgRNA2-ccdB-Cas9

Ori

Amp

araBAD

SpCas9

pST165

pUC19-sgRNA-ccdB

Ori

Amp

araBAD

pST166

BAD-Cas12-ccdB

oriV

Tet/TcR

araBAD

Beacon1

pST167

p11-LacY-sgRNA1-ccdB donor

Ori

Amp

araBAD

pST168

p11-LacY-sgRNA2-ccdB donor

Ori

Amp

araBAD

pST169

pUC19-2xcrRNA

Ori

Amp

pST170

pB025

repB

R6k

ErmG, Amp

P1

TetR

FnCas12a

pST171

pnCasPA-BEC

pRO1600 oriV

Ori, pRO1600 oriV

GmR

CBE(BE3)

pST172

pet28a-922，ok

Ori

Kan

J23119

IPTG

922Cas9

pST173

2-pet28a-ChCas12f1-DR

Ori

Kan

J23119

IPTG

ChCas12f1

pST174

3-pet28a-ChCas12f1-DR

Ori

Kan

J23119

IPTG

ChCas12f1

pST175

4-pet28a-ChCas12f1-tracr-DR

Ori

Kan

J23119

IPTG

ChCas12f1

pST176

4th_26

Ori

Kan

J23119

IPTG

pST177

PET28-0507a22

Ori

Kan

J23119

IPTG

pST178

pet28a-9

Ori

Kan

J23119

IPTG