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AsCas12a nuclease

AsCas12a Nuclease

Product Introduction

AsCas12a is a Type V-A CRISPR nuclease derived from the bacterium Acidaminococcus sp. BV3L6. Belonging to the Cas12a family alongside LbCas12a but originating from a different bacterial species, it exhibits unique advantages in the field of genome editing. This protein achieves double-stranded DNA cleavage through a single RuvC nuclease domain via a sequential cleavage mechanism: it first cleaves the non-target strand, then the target strand, ultimately generating approximately 5 nt 5' overhangs (sticky ends). This characteristic lends it particular value in applications such as directional gene insertion.

AsCas12a recognizes the T-rich PAM sequence 5'-TTTV-3' (where V represents A, C, or G). This feature enables it to effectively target AT-rich genomic regions that are difficult for SpCas9 to cover, thereby expanding the editable range across the genome. Studies have shown that AsCas12a exhibits excellent editing efficiency and high specificity in mammalian cells. At certain target sites, its editing efficiency can even surpass that of the most widely used LbCas12a, making it an important supplementary tool for researchers when selecting target sites.

Product Specifications

Parameter	Specification
Source	Recombinant expression in E. coli
Molecular Weight	~152 kDa
Concentration	20 μM
PAM Sequence	5'-TTTV-3'
Cleavage Site	18-23 nt downstream of PAM
Cleavage Product	5' sticky ends
Purity	≥95% (SDS-PAGE)
Endotoxin	<1 EU/μg
Storage Buffer	10 mM Tris-HCl, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol, pH 7.5
10× Reaction Buffer	10 mM Tris-HCl, 50 mM NaCl, 10 mM MgCl₂, 100 μg/ml BSA, pH 7.9
Storage Conditions	Long-term storage at -80°C; short-term storage at -20°C