MS2-p65-HSF1-mCherry Transcriptional Activation Helper Protein

Product Introduction

MS2-p65-HSF1-mCherry is a key auxiliary protein component within the Synergistic Activation Mediator (SAM) CRISPRa system. Built upon the MS2-p65-HSF1 framework, it incorporates the mCherry red fluorescent protein, achieving the dual functionality of potent transcriptional activation and real-time visualization tracking. This fusion protein consists of three parts: the MS2 phage coat protein, responsible for specifically recognizing MS2 aptamer sequences within RNA stem-loops; the NF-κB p65 activation domain and the Heat Shock Factor 1 (HSF1) activation domain, which provide two independent and potent transcriptional activation modules; and mCherry, serving as a fluorescent reporter tag characterized by high brightness, excellent photostability, and a spectrum that does not overlap with GFP.