CREG1 Protein (Cellular Repressor of E1A-Stimulated Genes 1）
Product Introduction
CREG1 (Cellular Repressor of E1A-Stimulated Genes 1) is an evolutionarily conserved glycoprotein of human origin, initially cloned and identified from a Drosophila cDNA library by yeast two-hybrid screening. The gene is located on human chromosome 1q24.2, with a full-length mRNA of 2048 bp containing 4 exons. The human CREG1 protein consists of 220 amino acids with a molecular weight of approximately 24.1 kDa (mouse CREG1 is approximately 24.5 kDa). This protein belongs to the CREG family (which includes two homologous proteins, CREG1 and CREG2), with CREG1 being ubiquitously expressed while CREG2 expression is restricted to brain tissue.
CREG1 was initially described as a transcriptional repressor capable of antagonizing the transcriptional activation and cellular transformation induced by the adenoviral E1A oncoprotein. The protein shares limited sequence similarity with E1A and binds in vitro to two key proteins: the general transcription factor TBP and the tumor suppressor protein pRb. With further research, the functional scope of CREG1 has been continuously expanded; it functions both as a lysosomal protein regulating endocytic pathways and as a secreted ligand involved in intercellular communication.
Product Specifications
Parameter | Specification |
Source | Recombinant expression in E. coli |
Molecular Weight | ~21 kDa |
Concentration | 1 mg/ml |
Purity | ≥95% (SDS-PAGE) |
Endotoxin | <1 EU/μg |
Storage Buffer | 50 mM Tris-HCl, 500 mM NaCl, 50% Glycerol, pH 8.0 |
10× Reaction Buffer | 200 mM Tris-HCl, 1 M NaCl, 1 mM EDTA, pH 7.5 |
Storage Conditions | Long-term storage at -80°C; short-term storage at -20°C |
Product Sizes
Specifications | Catalog Number | Concentration | Volume |
50 μL | GR300601 | 1 mg/mL | 50 μL |
250 μL | GR300602 | 1 mg/mL | 250 μL |
500 μL | GR300603 | 1 mg/mL | 500 μL |
Application Scenarios
Cardiovascular Disease Research:
CREG1 is highly expressed in the heart and plays a critical role in cardioprotection.
Inhibition of Myocardial Fibrosis: CREG1 recombinant protein attenuates myocardial fibrosis after myocardial infarction by suppressing the phenotypic switch of cardiac fibroblasts.
Autophagy Activation and Cardioprotection: CREG1 is a key regulator of cardiac autophagy. It protects the heart from nutrient stress-induced injury and alleviates age-related cardiac hypertrophy, fibrosis, and diastolic dysfunction by activating autophagy. CREG1 knockout mice exhibit cardiomyocyte damage under nutrient stress, while CREG1 overexpression restores autophagic flux.
Alleviation of Drug-Induced Cardiotoxicity: CREG1 reduces necroptosis by inhibiting the TYK2-STAT1 pathway, alleviating sorafenib-induced cardiotoxicity; it also attenuates doxorubicin-induced cardiotoxicity by inhibiting cardiomyocyte ferroptosis.
Inhibition of Atherosclerosis: CREG1 reduces atherosclerotic lesions in ApoE−/− mice by suppressing macrophage inflammation and promoting autophagy and lysosomal biogenesis.
Reference
1.Kobayashi, S., et al. (2021). CREG1 promotes lysosomal biogenesis and function. Autophagy, 17(12), 4249–4265.
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