Mammalian Cell Protein Expression and Purification

Mammalian protein expression systems excel at producing antibodies and proteins that require proper folding and post-translational modifications. However, transient expression systems in mammalian cells often suffer from limited yields, frequently necessitating scale-up to meet experimental demands, which increases both time and cost. Leveraging years of experience in protein expression, GeneRulor empowers clients to rapidly advance their functional, structural, and therapeutic research.

Service Overview

(1) Transient Transfection Expression: Utilizing advanced transfection technology to rapidly obtain target proteins with a short turnaround time, suitable for small-scale expression validation. Supports HEK293F cell lines.

(2) Stable Cell Line Construction: Establishment of high-expression stable cell lines suitable for large-scale production. Provides comprehensive services including monoclonal selection and expression optimization.

(3) Protein Purification: Multiple purification strategies, including tag-affinity purification and ion exchange chromatography, ensuring protein activity and purity.

Technical Advantages

The core strength of eukaryotic protein expression and purification systems lies in their ability to produce active proteins that closely resemble their native state in both structure and function. This is primarily attributed to their robust post-translational modification capabilities and sophisticated protein folding machinery.