Engineered Microbial Strain Construction

Engineered strains are microbial chassis developed through rational design and genetic modification. They play an indispensable role in biotechnology, industrial production, pharmaceutical R&D, and fundamental biological research. Common model chassis and industrial strains include Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae, and Corynebacterium glutamicum, as well as various non-conventional chassis with unique metabolic pathways, such as Pseudomonas putida and Yarrowia lipolytica.

These engineered microbes offer highly customizable physiological and metabolic traits tailored to specific applications:

Escherichia coli (Prokaryotic Model): As the most widely used genetic engineering host, E. coli boasts a well-defined genetic background and high heterologous expression efficiency. It remains central to large-scale recombinant protein production, metabolic pathway reconstruction, and synthetic biology component testing.

Yeasts (Eukaryotic Chassis): Including S. cerevisiae and non-conventional yeasts, these platforms offer robust eukaryotic post-translational modifications and flexible metabolic regulation. They are ideal for the biosynthesis of high-value natural products, the construction of "cell factories," food fermentation, and pharmaceutical intermediate production.

Bacillus subtilis (Secretory Host): Known for its superior protein secretion capabilities and simple fermentation requirements, it is essential for the high-efficiency synthesis of industrial enzymes (e.g., amylases), fine chemicals, and industrial biotechnology.

Live Biotherapeutic Products (LBPs): Engineered strains such as attenuated Salmonella and engineered probiotics serve as vital tools in medicine. They provide key platforms for targeted delivery mechanisms, microbiome intervention, and host-microbe interaction studies, paving the way for novel live-cell therapies and precision medicine.

Specialized Environmental Strains: Strains engineered for stress resistance or phage resistance offer diversified value in complex fermentation, industrial waste utilization, and agricultural biotech. These are used for high-efficiency biomanufacturing, platform compound production, and environmental pollutant degradation.

Our Service Advantages

(1) High Efficiency & Precision:

Utilizing CRISPR/Cas systems and high-throughput DNA assembly, we achieve exceptional genome integration and regulation efficiency. For most chassis, single-gene editing or large-fragment knock-in efficiency is significantly enhanced—approaching 100% in certain models. Our precise regulatory element design minimizes metabolic burden and off-target effects.

(2) Versatility & Customization:

We support diverse construction strategies, including gene knockouts, multi-site knock-ins, pathway reconstruction, and expression fine-tuning (e.g., promoter replacement). We offer custom expression vectors, metabolic flux redirection, and dynamic switches (e.g., light-controlled or small-molecule induced) to match the physiological characteristics of different strains.

(3) Broad Applicability:

Our services cover a wide range of prokaryotic chassis (E. coli, Bacillus, etc.) and eukaryotic chassis (Yeasts and fungi). We work with both standard laboratory model strains and promising industrial wild-type strains, supporting marker-free genome editing and stable clonal screening.

(4) Controllable Timelines:

Standard project cycles for chassis construction typically range from 1 to 4 months (1–2 months for routine model strains; 2–4 months for complex metabolic pathway reconstruction or non-conventional chassis). Our standardized workflows ensure reliable and reproducible results.