Silanol-group DNA extraction magnetic beads are high-performance magnetic microspheres specially designed for nucleic acid extraction and purification. With superparamagnetic Fe₃O₄ as the core, the microspheres are uniformly coated with silicon dioxide (SiO₂) on the outer layer, and modified with a large number of silanol (-OH) groups on the surface. This unique structure enables them to specifically bind to nucleic acids in solution under high-salt and low-pH conditions, efficiently adsorbing DNA or RNA through hydrophobic interaction, hydrogen bonding and electrostatic interaction, without non-specific binding to proteins, polysaccharides and other impurities.
These magnetic beads exhibit excellent magnetic responsiveness and dispersibility, enabling rapid solid-liquid separation under a magnetic field with simple and efficient operation. Abundantly modified with silanol groups on the surface, they feature strong nucleic acid binding capacity and high elution efficiency. The nucleic acids extracted with these beads have superior purity and can be directly used for downstream molecular biology experiments such as PCR, qPCR and sequencing. The product shows minimal batch-to-batch variation and stable performance, suitable for both manual operation and automated extraction instruments. It is widely applicable for nucleic acid extraction from various biological samples including blood, tissues, saliva and viral samples, providing reliable support for molecular diagnosis, forensic identification and life science research.
- Product Description
- Product Information
- Product Manual
Silanol-Group DNA Extraction Magnetic Beads
1. Product Advantages
(1) High nucleic acid binding capacity: Strong affinity for nucleic acids, with each milligram of magnetic beads capable of binding more than 20 μg of DNA.
(2) Excellent operational performance: Uniformly dispersed microspheres with superparamagnetism and a magnetic response time of less than 30 seconds.
(3) Superb batch stability: The deviation of PCR results for nucleic acids extracted with magnetic beads from different batches is less than 5%.
(4) High structural stability: Complete silica coating ensures consistent and stable performance during application.
2. DNA Extraction Protocol
(1) Sample preparation: Collect and properly store samples to ensure their suitability for DNA extraction.
(2) Cell lysis: Lyse cells with lysis buffer to release intracellular DNA.
(3) Bead-DNA binding: Mix magnetic beads with the lysed sample to facilitate the binding of DNA to the bead surface.
(4) Magnetic separation: Separate the magnetic beads using amagnetic rack and remove unbound impurities.
(5) Washing: Wash the magnetic beads to further removere sidual impurities.
(6) DNA elution: Elute DNA from the magnetic bead surface with elution buffer.
(7) DNA storage: Store the extracted DNA appropriately asrequired.
Figure 1. Schematic workflow of DNA extraction
Figure 2. Silanol-group nucleic acid extraction magnetic beads for internal application in automated nucleic acid extraction
Figure 3. Agarose gel electrophoresis of genomic DNA from HEK 293T cells extracted with silanol-group magnetic beads
Table 1. Concentration and purity of genomic DNA from HEK 293T cells extracted with silanol-group magnetic beads
ID | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
DNA Concentration 1 (ng/μL) | 151.9 | 157.4 | 126.9 | 193.7 | 99 | 117.9 | 177.8 |
A260/A280 | 1.92 | 1.92 | 1.90 | 1.87 | 1.84 | 1.91 | 1.92 |
DNA Concentration 2 (ng/μL) | 65.9 | 116.9 | 108 | 93.5 | 97.4 | 119.2 | 102.6 |
A260/A280 | 1.93 | 1.92 | 1.91 | 1.91 | 1.92 | 1.91 | 1.92 |
3. Precautions
(1) This product is for research use only. Please read this manual carefully before use.
(2) All reagents shall be used in accordance with the specifications after being taken out from the designated storage environment. Shake the reagents thoroughly to ensure uniform mixing before use.
(3) For accurate sampling, a micropipette is recommended for each sample addition step.
(4) Avoid direct contact of all samples and reagents with skin andeyes, and do not swallow. In case of accidental contact, rinse the affectedarea with plenty of water immediately and seek medical attention promptly.
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