pA-GpC Methyltransferase (pA-M.CviPI)

Product Introduction

GpC Methyltransferase (M.CviPI) is a DNA methyltransferase derived from Chlorella virus P1, belonging to the 5-methylcytosine (5mC) modification enzyme family. This enzyme was originally cloned and identified from the NYs-1 strain of Chlorella virus. The naturally encoded protein consists of 362 amino acids with a molecular weight of approximately 41.9 kDa.

Product Specifications

Product Specifications

Application Scenarios

DNA Physical Property and Structural Studies:

Z-DNA Inhibition: Methylation of GC sequences can reduce the free energy required for Z-DNA formation, influencing the degree of DNA helicity and dynamic properties.

Sequencing Optimization: In chemical sequencing, 5-methylcytosine exhibits reduced reactivity with hydrazine, facilitating the identification of methylation sites.

Genomic DNA Methylation and Epigenetics Research:

M.CviPI is widely used for in vitro methylation modification of genomic DNA, supporting research into the regulatory effects of methylation on gene expression. For example, combining methylated whole-genome DNA with high-throughput sequencing allows for the analysis of relationships between methylation patterns and diseases.

Molecular Cloning and Restriction Enzyme Protection:

Methylation modification can block the cleavage activity of restriction enzymes that recognize GC sequences (e.g., HaeIII). This is useful for avoiding non-specific cleavage during plasmid construction or PCR product processing.

Biosensors and Labeling Techniques:

When combined with [³H] labeling technology, M.CviPI enables uniform labeling of DNA for applications such as radiotracing or molecular interaction studies.

References

Walter JJ, et al. (2004). Mapping chromatin structure in vivo using DNA methyltransferases. Methods. 33(1):68-80.