Mouse Cot-1 DNA

1. Product Introduction

Mouse Cot‑1DNA is extracted from mouse liver DNA, with most fragments ranging from 50 to 300 bp. It is rich in repetitive DNA sequences, such as B1, B2, and L1 family members. Mouse Cot‑1 DNA is commonly used to block non‑specific hybridization in microarray screening. It can also be used to suppress hybridization of rodent repetitive DNA sequences (when mapping rodent clones by in situ hybridization or Southern blotting) and to identify mouse clonesin somatic cell hybrid library screening derived from mouse‑hamster hybrid cells.

2. Applications

Mouse Cot-1 DNA is mainly used in Northern blotting, Southern blotting, in situ hybridization (tissue In situ hybridization, somatic cell In situ hybridization, fluorescence In situ hybridization), and liquid-phase hybridization capture. In addition, Mouse Cot-1 DNA can also be used as a library screening probe for the screening of somatic cell hybrid libraries and flow-sorted chromosome libraries constructed from somatic cell hybrids.

Figure 1. Application scenarios of Mouse Cot‑1 DNA

References

[1] Bao P, Frutos A. G, Greef C .et al.Anal Chem (2002) 74:1792-1799.

[2] Tang Y M, Wo Y Y,Stewart J,.et al.JBiol Chem (1996) 271:28324-28354.

[3] Trifonov V.A, Vorobieva N.N, RensW. Springer (2009): FISH With and Without COT1 DNA.