Human Cot-1 DNA Pro

1. Product Introduction

Human Cot-1 DNA  Pro is a highly purified preparation of human genomic highly repetitive sequences approximately 50–300 bp in length. It can conveniently and efficiently block the repetitive sequences in probes or nucleic acid sequences of test samples, thereby avoiding non-specific hybridization signals during nucleic acid hybridization, improving hybridization performance, and ultimately achieving effective capture of target nucleic acid sequences, thus functioning as a blocking reagent. Experiments demonstrate that Human Cot-1 DNA Pro reduces false-positive signals caused by repetitive sequences and lowers background hybridization signals in chromosomal in situ hybridization, leading to more accurate and reliable experimental results. Most importantly, Human Cot-1 DNA Pro has smaller and more concentrated fragment sizes than conventional Human Cot-1 DNA, enabling better suppression of non-specific DNA binding.

Figure 1. Lanes 1, 2 and 3: Human Cot‑1DNA Pro; Lanes 4 and 5: Conventional Human Cot‑1DNA.

2. Applications

Human Cot-1 DNA Pro is mainly used in Northern blotting, Southern blotting, in situ hybridization (tissue In situ hybridization, somatic cell In situ hybridization, fluorescence In situ hybridization), and liquid-phase hybridization capture. Inaddition, Human Cot-1 DNA Pro can also be used as a library screening probe for the screening of somatic cell hybrid libraries and flow-sorted chromosome libraries constructed from somatic cell hybrids.

Figure 2. Application scenarios of Human Cot‑1 DNA Pro

References

[1] Bao P, Frutos A. G, Greef C .et al.Anal Chem (2002) 74:1792-1799.

[2] Tang Y M, Wo Y Y,Stewart J,.et al.JBiol Chem (1996) 271:28324-28354.

[3] Trifonov V.A, Vorobieva N.N, RensW. Springer (2009): FISH With and Without COT1 DNA.