Human Cot-1 DNA is a highly purified preparation of human genomic highly repetitive sequences approximately 50–300 bp in length. It acts as a core blocking reagent for a wide range of nucleic acid hybridization assays, including Northern blotting, Southern blotting, and liquid-phase hybridization capture. This reagent efficiently and conveniently blocks repetitive sequences in the nucleic acid sequences of probes and test samples, masks common repetitive sequence families such as Alu and LINE, and eliminates non-specific signal interference during hybridization. In doing so, it enhances hybridization specificity, enables the effective capture of target nucleic acid sequences, and fulfills the critical blocking function. Generulor Co., Ltd.’s Human Cot-1 DNA is manufactured using a proprietary fragmentation process, which ensures that over 50% of the fragments fall within the optimal 50–300 bp range, delivering a blocking efficiency of more than 95%. Rigorously compliant with stringent quality control standards and exhibiting high batch-to-batch consistency, this product significantly boosts capture efficiency, reduces sequencing data output, and cuts experimental costs, thereby providing robust and reliable support for all types of nucleic acid hybridization experiments.
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Human Cot-1 DNA
1.Applications of Human Cot-1 DNA
Human Cot-1 DNA is a highly purified preparation of human genomic highly repetitive sequences approximately 50–300 bp in length. It can conveniently and effectively block there petitive sequences present in probe or sample nucleic acids, thereby suppressing non-specific hybridization signals during nucleic acid hybridization. This improves overall hybridization performance and enables efficient capture of target sequences in the sample, fulfilling the key blocking function.
Figure 1. Schematic diagram of non‑specific DNA sequence blocking by Human Cot‑1 DNA
2.Applications of Human Cot-1 DNA
The major applications of Human Cot-1 DNA include Northern blotting, Southern blotting, In situ hybridization (tissue In situ hybridization, somatic cell In situ hybridization, fluorescence In situhybridization), and liquid-phase hybridization capture. In addition, Human Cot-1 DNA can be used as a library screening probe for somatic cell hybrid libraries and flow-sorted chromosome libraries constructed from somatic cell hybrids.
Figure 2. Application scenarios of Human Cot‑1 DNA
References
[1] Bao P, Frutos A. G, Greef C .et al. Anal Chem (2002) 74:1792-1799.
[2] Tang Y M, Wo Y Y,Stewart J,.et al.J Biol Chem (1996) 271:28324-28354.
[3] Trifonov V.A, Vorobieva N.N, Rens W. Springer (2009): FISH With and Without COT1 DNA.
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